Search | Engineering

Subscribe Submit

Home Journals Focus Achievement Fronts About Us 中文版

Resource Type

Journal Article 3

Year

2022 1

2016 1

Keywords

Salmonella VNP20009 1

B16F10 1

CFAP54 1

Distributed rule store 1

Multi-controller 1

Security 1

Software-defined networking (SDN) 1

angiogenesis 1

apoptosis 1

cilia 1

melanoma 1

primary ciliary dyskinesia 1

tumstatin 1

open ︾

Search scope:

排序： Display mode:

Lack of CFAP54 causes primary ciliary dyskinesia in a mouse model and human patients

Frontiers of Medicine doi: 10.1007/s11684-023-0997-7

Abstract: FAP54, the homolog of CFAP54 in Chlamydomonas reinhardtii, was previously demonstratedA Cfap54 knockout mouse model was then reported to have PCD-relevant phenotypes.T2438delinsTQAEFLA) in a new suspected PCD-relevant gene, CFAP54, were identified in an individualP2187S), in CFAP54 were detected in another unrelated patient.first time provides strong supportive evidence that CFAP54 is a new PCD-causing gene.

Keywords： primary ciliary dyskinesia CFAP54 cilia

HTML PDF Collect

Bacteria-mediated tumor-targeted delivery of tumstatin (54-132) significantly suppresses tumor growth

Frontiers of Medicine 2022, Volume 16, Issue 6, Pages 873-882 doi: 10.1007/s11684-022-0925-2

Abstract: Tumor growth is an angiogenesis-dependent process and accompanied by the formation of hypoxic areas. Tumstatin is a tumor-specific angiogenesis inhibitor that suppresses the proliferation and induces the apoptosis of tumorous vascular endothelial cells. VNP20009, an attenuated Salmonella typhimurium strain, preferentially accumulates in the hypoxic areas of solid tumors. In this study, a novel Salmonella-mediated targeted expression system of tumstatin (VNP-Tum5) was developed under the control of the hypoxia-induced J23100 promoter to obtain anti-tumor efficacy in mice. Treatment with VNP-Tum5 effectively suppressed tumor growth and prolonged survival in the mouse model of B16F10 melanoma. VNP-Tum5 exhibited a higher efficacy in inhibiting the proliferation and inducing the necrosis and apoptosis of B16F10 cells in vitro and in vivo compared with VNP (control). VNP-Tum5 significantly inhibited the proliferation and migration of mouse umbilical vascular endothelial cells to impede angiogenesis. VNP-Tum5 downregulated the expression of anti-vascular endothelial growth factor A, platelet endothelial cell adhesion molecule-1, phosphorylated phosphoinositide 3 kinase, and phosphorylated protein kinase B and upregulated the expression of cleaved-caspase 3 in tumor tissues. This study is the first to use tumstatin-transformed VNP20009 as a tumor-targeted system for treatment of melanoma by combining anti-tumor and anti-angiogenic effects.

Keywords： Salmonella VNP20009 tumstatin B16F10 melanoma apoptosis angiogenesis

HTML PDF Collect

Central Universities, China, the Program for New Century Excellent Talents in University, and the CETC 54 Article

Huan-zhao WANG,Peng ZHANG,Lei XIONG,Xin LIU,Cheng-chen HU

Frontiers of Information Technology & Electronic Engineering 2016, Volume 17, Issue 7, Pages 634-646 doi: 10.1631/FITEE.1500321

Abstract: Controllers play a critical role in software-defined networking (SDN). However, existing singlecontroller SDN architectures are vulnerable to single-point failures, where a controller’s capacity can be saturated by flooded flow requests. In addition, due to the complicated interactions between applications and controllers, the flow setup latency is relatively large. To address the above security and performance issues of current SDN controllers, we propose distributed rule store (DRS), a new multi-controller architecture for SDNs. In DRS, the controller caches the flow rules calculated by applications, and distributes these rules to multiple controller instances. Each controller instance holds only a subset of all rules, and periodically checks the consistency of flow rules with each other. Requests from switches are distributed among multiple controllers, in order to mitigate controller capacity saturation attack. At the same time, when rules at one controller are maliciously modified, they can be detected and recovered in time. We implement DRS based on Floodlight and evaluate it with extensive emulation. The results show that DRS can effectively maintain a consistently distributed rule store, and at the same time can achieve a shorter flow setup time and a higher processing throughput, compared with ONOS and Floodlight.

Keywords： Software-defined networking (SDN) Security Multi-controller Distributed rule store